NASC

NASC ID: N628284

Name: SALK_128284

ABRC stock number: SALK_128284

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At5g23480 K19M13.11 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_128284.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G23490.1 (at chr 5 pos 7919237 (C/7919237-7919288) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 58, R 57, C 54 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350288.

SALKseq_128284.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G09890=P355 and AT2G09900.1 (around chr 2 pos 3747787 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416312.

SALKseq_128284.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G30550 and AT3G30560.1=P355 (at chr 3 pos 12151464 (W/12151404-12151464) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 58, R 57, C 54 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO343596.

SALKseq_128284.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G20920.1 (at chr 4 pos 11195095 (W/11195037-11195095) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 58, R 57, C 54 N 54. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO346941.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_128284

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_128284C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G09890 AT2G09900 AT3G30550 AT3G30560 AT4G20920 At5g23490 AT5G23490 At5g23480

EMBL accession:

BZ292761

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.