NASC Stock Detail Page
NASC ID: N628892
Name: SALK_128892
ABRC stock number: SALK_128892
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. January 2003 TAIR gene annotation - At3g50670 T3A5.50 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_128892.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G50670.1 (at chr 3 pos 18829551 (C/18829551-18829592) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 60, R 59, C 56 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373742. SALKseq_128892.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G19700.1=P355 and AT4G19710.1=P355 (at chr 4 pos 10718628 (W/10718550-10718628) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 60, R 59, C 56 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO377095. SALKseq_128892.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G26690.1=P355 and AT4G26700.2=P355 (at chr 4 pos 13460996 (W/13460902-13460996) on the TAIR10). The sequences' 1-95 bps mapped on the genome. Pools P 60, R 59, C 56 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO380442. SALKseq_128892.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G65700.2=UTR3 and AT5G65710.1 (at chr 5 pos 26290482 (C/26290482-26290530) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 44-79 bps onto the T-DNA. Pools P 60, R 59, C 56 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO383785. SALKseq_128892.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G16190.1 and AT5G16200.1=UTR3 (at chr 5 pos 5289198 (W/5289120-5289198) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 60, R 59, C 56 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387129.
Donation Date: 2003-01-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_128892
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_128892C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.