NASC

NASC ID: N629700

Name: SALK_129700

ABRC stock number: SALK_129700

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At4g00700 F6N23.8 E xon F6N23.8 E xon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_129700.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G00700.1 (at chr 4 pos 286424 (W/286347-286424) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 61, R 65, C 69 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO347014.

SALKseq_129700.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G47260.1=P355 and AT2G47270.1=UTR3 (at chr 2 pos 19409509 (C/19409509-19409554) on the TAIR10). The sequences' 34-79 bps mapped on the genome. Pools P 61, R 65, C 69 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410567.

SALKseq_129700.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G33004=P355 and AT3G33005.1=P355 (at chr 3 pos 13543610 (W/13543542-13543610) on the TAIR10). The sequences' 1-69 bps mapped on the genome, 71-95 bps onto the T-DNA. Pools P 61, R 65, C 69 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO413917.

SALKseq_129700.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G02880.1 (at chr 4 pos 1274201 (C/1274201-1274263) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 61, R 65, C 69 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO343670.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_129700

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N663977 (SALK_129700C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G47260 AT2G47270 AT3G33004 AT3G33005 AT4G02880 At4g00700

EMBL accession:

BZ356773

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.