NASC Stock Detail Page
NASC ID: N630978
Name: SALK_130978
ABRC stock number: SALK_130978
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At4g16900 FCAALL. 31 0 R300-5' FCAALL. 31 0 R300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_130978.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G16900.1 and AT4G16915.1 (at chr 4 pos 9516654 (C/9516654-9516739) on the TAIR10). The sequences' 1-87 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 58, R 53, C 59 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO343727. SALKseq_130978.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G17950.1=UTR3 and AT1G17960.1=UTR3 (at chr 1 pos 6180512 (C/6180512-6180593) on the TAIR10). The sequences' 1-82 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 58, R 53, C 59 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407281. SALKseq_130978.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G15400.1 (at chr 2 pos 6713125 (W/6713055-6713125) on the TAIR10). The sequences' 1-71 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 58, R 53, C 59 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410623. SALKseq_130978.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G16860.1 (around chr 4 pos 9495688 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416388.
Donation Date: 2003-03-11
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_130978
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_130978C.
| Status | Price (£) |
|---|---|
| Available | £10.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.