NASC

NASC ID: N631610

Name: SALK_131610

ABRC stock number: SALK_131610

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At5g09810 MYH9.2 Ex on MYH9.2 Ex on. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_131610.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G09810.1 (at chr 5 pos 3052891 (C/3052891-3052940) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 47, R 43, C 45 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403977.

SALKseq_131610.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G67710.1 (at chr 1 pos 25376960 (W/25376903-25376960) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 59-76 bps onto the T-DNA. Pools P 47, R 43, C 45 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393956.

SALKseq_131610.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G24710.1 (at chr 2 pos 10519360 (W/10519302-10519360) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 47, R 43, C 45 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397292.

SALKseq_131610.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G31660.1 (at chr 2 pos 13465606 (W/13465529-13465606) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 47, R 43, C 45 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400635.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_131610

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_131610C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G67710 AT2G24710 AT2G31660 At5g09810

EMBL accession:

BZ357949

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.