NASC

NASC ID: N631706

Name: SALK_131706

ABRC stock number: SALK_131706

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At2g13270 F15O11. 9 Intron F15O11. 9 Intron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_131706.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G13270.1 (at chr 2 pos 5493280 (W/5493242-5493280) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 36, R 36, C 35 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO377227.

SALKseq_131706.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G04940.1 (at chr 1 pos 1400554 (W/1400505-1400554) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 51-76 bps onto the T-DNA. Pools P 36, R 36, C 35 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO367174.

SALKseq_131706.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G77525.1=P355 and AT1G77530.1 (at chr 1 pos 29135430 (W/29135381-29135430) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 48-67 bps onto the T-DNA. Pools P 36, R 36, C 35 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO370522.

SALKseq_131706.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G36380.1 (at chr 2 pos 15262752 (W/15262722-15262752) on the TAIR10). The sequences' 1-31 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 36, R 36, C 35 N 34. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373873.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_131706

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_131706C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G04940 AT1G77525 AT1G77530 AT2G36380 At2g13270

EMBL accession:

BZ357986

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.