NASC Stock Detail Page
NASC ID: N631836
Name: SALK_131836
ABRC stock number: SALK_131836
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_131836.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G16870.1 (at chr 5 pos 5548896 (C/5548896-5548955) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 14, R 16, C 13 N 16. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO357129. SALKseq_131836.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G47350.1 and AT1G47360.1=P355 (at chr 1 pos 17360825 (W/17360747-17360825) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 14, R 16, C 13 N 16. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO343766. SALKseq_131836.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G47360 and AT1G47370.1 (at chr 1 pos 17365448 (W/17365370-17365448) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 14, R 16, C 13 N 16. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO347110. SALKseq_131836.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G51020.1=UTR3 and AT1G51030.1=P355 (at chr 1 pos 18914686 (C/18914686-18914749) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 14, R 16, C 13 N 16. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350457. SALKseq_131836.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G33370.2=P355 and AT5G33380.1=P355 (at chr 5 pos 12606082 (W/12606020-12606082) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 14, R 16, C 13 N 16. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353801.
Donation Date: 2003-03-11
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_131836
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_131836C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.