NASC

NASC ID: N631854

Name: SALK_131854

ABRC stock number: SALK_131854

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At1g53590 F22G10. 28 Exon F22G10. 28 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_131854.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G53590.1 (at chr 1 pos 20000029 (C/20000029-20000079) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 51-79 bps onto the T-DNA. Pools P 18, R 18, C 14 N 12. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400648.

SALKseq_131854.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G48950.1 (at chr 3 pos 18148202 (W/18148143-18148202) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 18, R 18, C 14 N 12. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403989.

SALKseq_131854.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G37235.1=UTR3 and AT4G37240.1=P355 (at chr 4 pos 17520207 (W/17520129-17520207) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 18, R 18, C 14 N 12. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407320.

SALKseq_131854.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G43470.1 (at chr 5 pos 17466276 (W/17466216-17466276) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 59-76 bps onto the T-DNA. Pools P 18, R 18, C 14 N 12. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410664.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_131854

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_131854C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G48950 AT4G37235 AT4G37240 AT5G43470 At1g53590

EMBL accession:

BZ358076

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.