NASC

NASC ID: N632063

Name: SALK_132063

ABRC stock number: SALK_132063

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At3g25805 K13N2.1 6 L300-3' K13N2.1 6 L300-3'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_132063.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G25800.1 (at chr 3 pos 9426189 (W/9426110-9426189) on the TAIR10). The sequences' 1-80 bps mapped on the genome, 82-100 bps onto the T-DNA. Pools P 25, R 22, C 22 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO340406.

SALKseq_132063.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G62750.1 (at chr 1 pos 23236373 (W/23236306-23236373) on the TAIR10). The sequences' 1-68 bps mapped on the genome. Pools P 2, R 2, C 1 N 7. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429883.

SALKseq_132063.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G42290.1 (at chr 5 pos 16911511 (W/16911456-16911511) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 52-76 bps onto the T-DNA. Pools P 2, R 2, C 1 N 7. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433188.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_132063

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_132063C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G62750 At3g25800 AT3G25800 AT5G42290 At3g25805

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.