NASC

NASC ID: N633134

Name: SALK_133134

ABRC stock number: SALK_133134

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At2g35530 T32F12. 9 L300-3' T32F12. 9 L300-3'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_133134.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G12672.2 (at chr 1 pos 4318078 (C/4318078-4318156) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 9, R 7, C 4 N 1. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353848.

SALKseq_133134.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G07570 and AT4G07580.1=P355 (at chr 4 pos 4390940 (W/4390879-4390940) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 60-77 bps onto the T-DNA. Pools P 9, R 7, C 4 N 1. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO357174.

SALKseq_133134.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G08054.1 (at chr 4 pos 4920035 (W/4919957-4920035) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 9, R 7, C 4 N 1. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360529.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_133134

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_133134C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G12672 AT4G07570 AT4G07580 AT4G08054 At2g35530

EMBL accession:

BZ358666

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.