NASC Stock Detail Page
NASC ID: N633518
Name: SALK_133518
ABRC stock number: SALK_133518
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_133518.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G29033.1 and AT4G29035.1 (at chr 4 pos 14309915 (W/14309838-14309915) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 46, R 48, C 49 N 49. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO394034. SALKseq_133518.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G10990 and AT4G11000.1 (around chr 4 pos 6729988 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416451. SALKseq_133518.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G45250.1 (at chr 5 pos 18325247 (W/18325194-18325247) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 59-76 bps onto the T-DNA. Pools P 46, R 48, C 49 N 49. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400713. SALKseq_133518.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G62630.1=P355 and AT5G62640.1=UTR3 (at chr 5 pos 25148011 (W/25147935-25148011) on the TAIR10). The sequences' 1-77 bps mapped on the genome. Pools P 46, R 48, C 49 N 49. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO404053. SALKseq_133518.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G25400.1 and AT5G25410.1=P355 (at chr 5 pos 8825974 (W/8825925-8825974) on the TAIR10). The sequences' 3-52 bps mapped on the genome, 52-74 bps onto the T-DNA. Pools P 46, R 48, C 49 N 49. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407383.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_133518
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_133518C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.