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NASC ID: N634057

Name: SALK_134057

ABRC stock number: SALK_134057

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At3g16880 MUH15.4 L 300-5' MUH15.4 L 300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_134057.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G16870.1=P355 and AT3G16880.1 (at chr 3 pos 5766415 (C/5766415-5766460) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 52-79 bps onto the T-DNA. Pools P 56, R 54, C 57 N 51. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400744.

SALKseq_134057.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G75410.1=P355 and AT1G75420.1=P355 (at chr 1 pos 28303744 (W/28303666-28303744) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 56, R 54, C 57 N 51. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390706.

SALKseq_134057.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G13160.1 (at chr 1 pos 4487189 (C/4487189-4487236) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 45-70 bps onto the T-DNA. Pools P 56, R 54, C 57 N 51. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO394064.

SALKseq_134057.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G50010.1=P355 and AT3G50020.1 (at chr 3 pos 18541772 (W/18541721-18541772) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 56, R 54, C 57 N 51. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397400.

Donation Date: 2003-03-11

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_134057

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664057 (SALK_134057C).

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.