NASC

NASC ID: N636906

Name: SALK_136906

ABRC stock number: SALK_136906

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At3g62430 T12C14. 13 0 Exon T12C14. 13 0 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_136906.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G62430.1 (at chr 3 pos 23095289 (W/23095229-23095289) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 79, R 76, C 76 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360707.

SALKseq_136906.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G67120.1 (at chr 1 pos 25082220 (C/25082220-25082249) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 27-60 bps onto the T-DNA. Pools P 79, R 76, C 76 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350683.

SALKseq_136906.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G31317.1 (at chr 3 pos 12685999 (W/12685949-12685999) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 79, R 76, C 76 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO354028.

SALKseq_136906.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G54910.1 (at chr 3 pos 20345026 (W/20344969-20345026) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 79, R 76, C 76 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO357354.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_136906

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_136906C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G67120 AT3G31317 AT3G54910 At3g62430

EMBL accession:

BZ766194

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.