NASC

NASC ID: N638712

Name: SALK_138712

ABRC stock number: SALK_138712

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At3g57920 T10K17. 13 0 Intron T10K17. 13 0 Intron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_138712.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G57920.1 (at chr 3 pos 21445431 (W/21445371-21445431) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 7, R 8, C 3 N 4. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400967.

SALKseq_138712.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G40127=P355 and AT1G40129.1 (at chr 1 pos 15227753 (C/15227753-15227799) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 44-79 bps onto the T-DNA. Pools P 7, R 8, C 3 N 4. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390929.

SALKseq_138712.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G40127=P355 and AT1G40129.1 (at chr 1 pos 15230226 (W/15230148-15230226) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 7, R 8, C 3 N 4. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO394282.

SALKseq_138712.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G40127=P355 and AT1G40129.1 (at chr 1 pos 15234207 (C/15234207-15234285) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 7, R 8, C 3 N 4. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397624.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_138712

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_138712C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G40127 AT1G40129 At3g57920

EMBL accession:

BZ767341

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.