NASC Stock Detail Page
NASC ID: N639293
Name: SALK_139293
ABRC stock number: SALK_139293
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At4g37705 T28I19. 5 Exon T28I19. 5 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_139293.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G40085 and AT1G40087.1=UTR3 (around chr 1 pos 14998932 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO416611. SALKseq_139293.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G62410.1 and AT1G62421.1 (at chr 1 pos 23096115 (W/23096062-23096115) on the TAIR10). The sequences' 4-57 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 6, R 10, C 5 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO364181. SALKseq_139293.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G14247.1 (at chr 2 pos 6034395 (C/6034395-6034449) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 52-75 bps onto the T-DNA. Pools P 6, R 10, C 5 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO367528. SALKseq_139293.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G47360.1 and AT3G47370.1=UTR3 (at chr 3 pos 17453223 (C/17453223-17453282) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 6, R 10, C 5 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO370875. SALKseq_139293.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G06610.1 (at chr 3 pos 2061332 (W/2061275-2061332) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 6, R 10, C 5 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO374224.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_139293
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_139293C.
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.