NASC

NASC ID: N639401

Name: SALK_139401

ABRC stock number: SALK_139401

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At2g18140 F8D23.8 E xon F8D23.8 E xon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_139401.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G18130.1 and AT2G18140.1 (at chr 2 pos 7887309 (W/7887246-7887309) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 49, R 48, C 42 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360828.

SALKseq_139401.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G61350.1 (at chr 1 pos 22633790 (W/22633714-22633790) on the TAIR10). The sequences' 1-77 bps mapped on the genome, 76-95 bps onto the T-DNA. Pools P 49, R 48, C 42 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO354150.

SALKseq_139401.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G42000.1 (at chr 5 pos 16799350 (W/16799279-16799350) on the TAIR10). The sequences' 1-72 bps mapped on the genome, 67-95 bps onto the T-DNA. Pools P 49, R 48, C 42 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO364185.

SALKseq_139401.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G18130.1 and AT2G18140.1 (at chr 2 pos 7887309 (W/7887249-7887309) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 56-76 bps onto the T-DNA. Pools P 49, R 48, C 42 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO357481.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_139401

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_139401C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G61350 AT2G18130 AT5G42000 At2g18140

EMBL accession:

BZ767811

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.