NASC Stock Detail Page
NASC ID: N68100
Name: pMLO9::GUS
ABRC stock number: CS68100
Description: DNA fragment (covering 5' upstream of the AtMLO9 promoter region) from Col-0 was amplified and cloned into the SmaI site of pBluescript, then excised as EcoRI/NcoI and co-ligated with an NcoI/HindIII GUS fragment (excised from pSLJ4D4) into the binary vector pPZP211, and the Col-0 plants were transformed using the floral dip method. Homozygous for the transgene. Kanamycin resistant.
Donation Date: 2013-08-22
Donated by: | University of North Carolina, Chapel Hill Alan M. Jones |
Donor Number: pMLO9::GUS
Stock type: individual line
Material type: seed
Comment: Donors: Alan M. Jones. Parents: Col(Columbia)
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Seed type: Promoter reporter line
Background: Col(Columbia)
Growth requirement: none
Phenotype
Expressed in vascular tissues, petioles of the leaf and cotyledon, stem and in the the floral organs during or after the shedding process.
References
Chen, Z. et al. 2006. Expression analysis of the AtMLO gene family encoding plant-specific seven-transmembrane domain proteins. Plant Molecular Biology 60(4):583-97. PubMed ID: 16525893.