Home | About NASC | Address/Staff
Ask a question | Help

NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N68100

Name: pMLO9::GUS

ABRC stock number: CS68100

Description: DNA fragment (covering 5' upstream of the AtMLO9 promoter region) from Col-0 was amplified and cloned into the SmaI site of pBluescript, then excised as EcoRI/NcoI and co-ligated with an NcoI/HindIII GUS fragment (excised from pSLJ4D4) into the binary vector pPZP211, and the Col-0 plants were transformed using the floral dip method. Homozygous for the transgene. Kanamycin resistant.

Donation Date: 2013-08-22

Donated by:  University of North Carolina, Chapel Hill Alan M. Jones

Donor Number: pMLO9::GUS

Stock type: individual line

Material type: seed

Comment: Donors: Alan M. Jones. Parents: Col(Columbia)

Status Price (£)
Available £11.00

Germplasm Info

Seed type: Promoter reporter line

Background: Col(Columbia)

Growth requirement: none

Phenotype

Expressed in vascular tissues, petioles of the leaf and cotyledon, stem and in the the floral organs during or after the shedding process.

References

Chen, Z. et al. 2006. Expression analysis of the AtMLO gene family encoding plant-specific seven-transmembrane domain proteins. Plant Molecular Biology 60(4):583-97. PubMed ID: 16525893.