NASC Stock Detail Page
NASC ID: N69093
Name: meb1-1 meb2-1 amiRat4g27870-1
ABRC stock number: CS69093
Description: An amiR specific to At4g27870 (amiRat4g27870-1) was constructed. The constructed amiR was transferred into the binary vector pMDC7 to generate pMDC7/amiRat4g27870-1. This plasmid encodes the amiR gene under the control of the estradiol-inducible promoter. The plasmid was introduced into Agrobacterium GV3101 (pMP90RK) and then transformed into meb1-1 meb2-1 double mutants (CS69092). Homozygous for both mutations. Homozygous for the transgene.
Donation Date: 2015-12-15
| Donated by: | National Institute for Basic Biology - NINS Mikio Nishimura |
Donor Number: meb1-1 meb2-1 amiRat4g27870-1
Stock type: individual line
Material type: seed
Comment: Donors: Mikio Nishimura. Clone: amiRat4g27870-1.
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Seed type: Mutant
Background: Col(Columbia)
Growth requirement: 5 uM 17beta-estradiol is required for amiR expression.
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
The plants do not accumulate MEB1 and MEB2 proteins. Expression of a homologous gene, At4g27870 is reduced.
References
Yamada, K. et al. 2013. Identification of two novel endoplasmic reticulum body-specific integral membrane proteins. Plant Physiology 161(1):108-20.PubMed ID: 23166355