NASC Stock Detail Page
NASC ID: N6947
Name: til1-4 / til1-4
ABRC stock number: CS6947
Description: A line homozygous for the shoot apical meristem-expressed PSTM:GUS reporter (the promoter of the SHOOT MERISTEMLESS gene fused to beta-glucuronidase) was mutagenized with ethyl methanesulfonate and screened for embryo-defective mutants.
Donation Date: 2006-09-01
| Donated by: | University of Wisconsin, Madison Kathy Barton Franklin and Marshall College Pablo Jenik |
Donor Number: til1-4 / til1-4
Part of : Homozygous lines
Stock type: individual line
Material type: seed
Comment: gene name:TILTED 1, recessive mutation
| Status | Price (£) |
|---|---|
| Available | £10.00 |
Germplasm Info
Seed type: Mutant
Mutagen: ethylmethane sulfonate
Background: Col
Segregation status: homozygous
Pedigree: Backcrossed 3 times to Col.
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Weak allele. Embryos have altered placement of the root pole, slow embryonic development, longer cell cycles (~35%) and larger cells. Abnormal division of the hypophysis in the developing root of the embryo, generating a lens cell that is abnormal in shape or abnormally positioned; asymmetric development of the provasculature, this ultimately leads to an abnormal root pole which is displaced laterally causing the root-shoot axis to be at an angle to the long axis of the suspensor, giving the embryo a tilted appearance. Embryos are viable, have larger cells and nuclei than wild-type embryos but the same DNA content, mutant embryos are larger than wild-type embryos at the same developmental stage. Plants have slow growing roots, slightly delayed flowering, altered floral phyllotaxis, reduced number of ovules, abnormally developing ovules, and reduced fertility.