NASC Stock Detail Page
NASC ID: N70764
Name: ProCESA10:GUS
ABRC stock number: CS70764
Description: ProCESA10::GUS, transgenic line containing the cellulose synthase 10 (CESA10) promoter, E. coli B-glucuronidase (GUS) reporter gene; genomic fragments upstream of the CESA initiating AUG codon were amplified by PCR, cloned into pRITA upstream of the GUS gene, and sequenced to confirm accuracy of amplification; the promoter:GUS cassettes were then cloned at NotI fragments into the Ti plasmid pMLBART in the same orientation as the BAR gene, introduced into Agrobacterium strain ASE1 by electroporation, and used to transform Col-0 Arabidopsis by the floral dip method; transgenic plants were selected with a 1:1,000 dilution of Finale (Hoechst-Roussel Agri-Vet, Somerville, NJ) twice a week for three weeks; expression of GUS was confirmed by the standard histological assay at several subsequent generations.
Donation Date: 2008-02-14
| Donated by: | University of California, Davis Deborah Delmer |
Donor Number: ProCESA10:GUS
Stock type: individual line
Material type: seed
Comment: related stock N70755...N70764
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Organelle marker line
Background: Col-2(N907)
Phenotype
CESA10::GUS transgene expression initially detected in ovules.