NASC Stock Detail Page
NASC ID: N71321
Name: Penn_chr1_LR343
ABRC stock number: CS71321
Description: The napA promoter sequence, the dsRED open reading frame, and the nos sequence was PCR-amplified. After overlapping PCR, the NAP:dsRED-NOS expression cassette was cloned into the pCAMBIA3300 vector. The resulting plasmid was sequenced, after which, the Ler plant was transformed by using the floral dip method. Homozygous for the transgene. Basta resistant.
Donation Date: 2015-04-03
| Donated by: | University of Pennsylvania Scott Poethig |
Donor Number: Penn_chr1_LR343
Stock type: individual line
Material type: seed
Comment: NAP:dsRED expression cassette used. In publications involving this stock, please acknowledge the donor. Example template: The single insertion and traffic lines were a gift from Scott Poethig.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Background: Ler(Landsberg_erecta)
Growth requirement: none
Transformation method: Floral dip method
References
Wu, G. et al. 2015. Traffic lines: new tools for genetic analysis in Arabidopsis thaliana. Genetics 200(1):35-45. PubMed ID: 25711279.