NASC Stock Detail Page
NASC ID: N8049
Name: DsG1
ABRC stock number: CS8049
Description: Contains maize Dissociation (Ds) transposable element; gene trap (exon trap, intron trap) line; kanamycin resistant (NPTII); beta-glucuronidase (GUS) with intron splice acceptor sites in all three frames, no promoter; naphthalene acetamide (NAM) sensitive (contains indole acetic acid hydrolase, IAAH); crosses to Ac lines (CS8043, CS8044, and CS8045) induce transposition of Ds; selection for kanamycin resistance and NAM insensitivity in F2 generation results in transposed lines lacking Ac, and favors transpositions not closely linked to the donor Ds site; expression of GUS may occur if 5-prime end of GUS is transposed into an intron or in-frame in an exon; introduced into plants by Agrobacterium transformation - contains T-DNA borders.
Donation Date: 1996-06-20
| Donated by: | University of California, Davis Venkatesan Sundaresan Cold Spring Harbor Laboratory Rob Martienssen |
Donor Number: DsG1
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Gene trap
Mutagen: Ds Transposon Insertion
Background: Ler (Landsberg erecta)
Transformation method: Agrobacterium transformation
Photograph
References
Sundaresan, V. et al. 1995. Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements. Genes & Development 9(14):1797-810.PubMed ID: 7622040.