NASC Stock Detail Page
NASC ID: N850743
Name: WiscDsLox297300_04E
ABRC stock number: CS850743
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox297300_04E.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G72730.1 (chr 1 pos 27380052 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG798039. Wiscseq_DsLox297300_04E.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G30835 and AT1G30840.2-P355 (chr 1 pos 10971825 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG798038. Wiscseq_DsLox297300_04E.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G16620.1 and AT2G16630.1-P355 (chr 2 pos 7208303 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG798040. Wiscseq_DsLox297300_04E.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G50665 (chr 3 pos 18825901 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG798041.
Donation Date: 2004-12-01
Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox297300_04E
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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