NASC

NASC ID: N851817

Name: WiscDsLox339E01

ABRC stock number: CS851817

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox339E01 .

Wiscseq_DsLox339E01.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G44600.1 (chr 5 pos 17991389 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG799706.

Wiscseq_DsLox339E01.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G65480.1-UTR3 and AT1G65481.1-UTR3 (chr 1 pos 24336941 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG799705.

Wiscseq_DsLox339E01.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G71050.1-P355 and AT1G71060.1 (chr 1 pos 26805138 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG799704.

Donation Date: 2006-09-04

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox339E01

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox339E01	WiscDsLox339E01	AT1G65480 AT1G65481 AT1G71050 AT1G71060 At5g44600

EMBL accession:

CW799534