NASC Stock Detail Page
NASC ID: N853378
Name: WiscDsLox377-380E17
ABRC stock number: CS853378
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox377-380E17.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G25760.1 (at chr 3 pos 9404375 (C/9404375-9404456) on the TAIR10). The sequences' 1-82 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 4, R 0, C 6 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO453652. Wiscseq_DsLox377-380E17.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G46350.1-P355 and AT5G46360.2-UTR3 (at chr 5 pos 18805865 (C/18805865-18805943) on the TAIR10). The sequences' 1-79 bps mapped on the genome, 82-100 bps onto the T-DNA. Pools P 4, R 0, C 6 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452159.
Donation Date: 2004-12-01
Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox377-380E17
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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