NASC

NASC ID: N854067

Name: WiscDsLox390F09

ABRC stock number: CS854067

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox390F09 .

Wiscseq_DsLox390F09.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G44650.1 (chr 2 pos 18420607 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG802630.

Wiscseq_DsLox390F09.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G14650.1 (chr 1 pos 5027678 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG802629.

Wiscseq_DsLox390F09.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G32000.2-P355 and AT2G32010.1-P355 (at chr 2 pos 13622702 (C/13622702-13622784) on the TAIR10). The sequences' 1-83 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 4, R 7, C 1 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452482.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox390F09

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
		CHL-CPN10	AT1G14650 AT2G32000 AT2G32010 At2g44650

EMBL accession:

CW799847