NASC

NASC ID: N856608

Name: WiscDsLox453-456I2

ABRC stock number: CS856608

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox453-456I2 .

Wiscseq_DsLox453-456I2.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G21030 (chr 3 pos 7370698 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG806083.

Wiscseq_DsLox453-456I2.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G23740.1-P355 and AT3G23750.1 (chr 3 pos 8557482 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG806084.

Wiscseq_DsLox453-456I2.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G10060.1 (at chr 4 pos 6291593 (C/6291593-6291692) on the TAIR10). The sequences' 1-100 bps mapped on the genome, 81-98 bps onto the T-DNA. Pools P 7, R 1, C 1 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452807.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox453-456I2

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox453-456I2	WiscDsLox453	AT3G23740 AT3G23750 AT4G10060 At3g21030

EMBL accession:

CW797689