NASC Stock Detail Page
NASC ID: N869147
Name: SALK_105419.2
ABRC stock number: CS869147
Description: confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
Donation Date: 2014-04-28
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALKseq_105419.2
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Donors: Joseph R. Ecker. 2015-02-18 GenBank annotation -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G11460.1-P355 and AT5G11470.1-UTR3 (chr 5 pos 3661720 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. GenBank Acc: KG787677.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Background: Col-0(SALK_105419)
Segregation status: homozygous
Growth requirement: none