NASC Stock Detail Page
NASC ID: N874546
Name: SAIL_524_E11
ABRC stock number: CS874546
Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. Associated Polymorphisms: SAILseq_524_E11.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G11740.1 (at chr 5 pos 3784219 (C/3784219-3784256) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 52-95 bps onto the T-DNA. Pools P 11, R 14, C 14 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO329267.
Donation Date: 2006-06-21
Donated by: | Syngenta AG Alberto Martinez Syngenta AG Allen Sessions |
Donor Number: SAIL_524_E11
Part of : SAIL lines from Syngenta
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-0(N60000)
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Sessions, A. et al. 2002. A high-throughput Arabidopsis reverse genetics system. The Plant cell 14(12):2985-94.PubMed ID: 12468722.