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The European Arabidopsis Stock Centre

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NASC ID: N879492

Name: SAIL_521_H04

ABRC stock number: CS879492

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:

SAILseq_521_H04.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G24230.1 (at chr 4 pos 12568814 (W/12568773-12568814) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 64-95 bps onto the T-DNA. Pools P 12, R 18, C 15 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO330742.

SAILseq_521_H04.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G22340.1 (at chr 1 pos 7891420 (W/7891326-7891420) on the TAIR10). The sequences' 1-95 bps mapped on the genome. Pools P 12, R 18, C 15 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO338712.

SAILseq_521_H04.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G23750.1 (at chr 4 pos 12376126 (C/12376126-12376220) on the TAIR10). The sequences' 1-95 bps mapped on the genome. Pools P 12, R 18, C 15 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO324611.

SAIL_521_H04 .

Donation Date: 2006-06-21

Donated by:  Syngenta AG Alberto Martinez
Syngenta AG Allen Sessions

Donor Number: SAIL_521_H04

Part of : SAIL lines from Syngenta

Stock type: individual line

Material type: seed

Status Price (£)
Available to order at NASC £11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-0(N60000)

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

References

Sessions, A. et al. 2002. A high-throughput Arabidopsis reverse genetics system. The Plant cell 14(12):2985-94.PubMed ID: 12468722.