NASC Stock Detail Page
NASC ID: N879589
Name: SAIL_628_E05
ABRC stock number: CS879589
Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. Associated Polymorphisms: SAILseq_628_E05.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G03350.1 (at chr 2 pos 1020497 (W/1020443-1020497) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 12, R 18, C 17 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO339102.
Donation Date: 2006-06-21
Donated by: | Syngenta AG Alberto Martinez Syngenta AG Allen Sessions |
Donor Number: SAIL_628_E05
Part of : SAIL lines from Syngenta
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-0(N60000)
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Sessions, A. et al. 2002. A high-throughput Arabidopsis reverse genetics system. The Plant cell 14(12):2985-94.PubMed ID: 12468722.