NASC Stock Detail Page
NASC ID: N29912
Name: 3bhsd-2
ABRC stock number: CS29912
Description: CRISPR/Cas9 generated knockout of EcCYP87A126, the cytochrome P450 responsible for sterol side chain cleavage and the initiation of cardenolide biosynthesis in Erysimum cheiranthoides. One bp insertion in coding sequence of CYP87A126 (Erche04g002680) results in loss of function and complete loss of cardenolide accumulation in plant tissues. No obvious visual phenotype or special growth requirements.
Donation Date: 2024-05-24
| Donated by: | Cornell University Georg Jander |
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Genus: Erysimum Species: cheiranthoides
Seed type: Transgenic
Mutagen: CRISPR
Background: Elbtalaue
Segregation status: homozygous
Growth requirement: Stratification for 2 days at 4C recommended
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
5beta-cardenolide synthesis reduced, accumulation of delta5-cardenolides
References
Younkin GC, Alani ML, Züst T, Jander G.; 2024-04-11; Four enzymes control natural variation in the steroid core of Erysimum cardenolides.; bioRxiv; PubMed ID: 38645095