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The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N29912

Name: 3bhsd-2

ABRC stock number: CS29912

Description: CRISPR/Cas9 generated knockout of EcCYP87A126, the cytochrome P450 responsible for sterol side chain cleavage and the initiation of cardenolide biosynthesis in Erysimum cheiranthoides. One bp insertion in coding sequence of CYP87A126 (Erche04g002680) results in loss of function and complete loss of cardenolide accumulation in plant tissues. No obvious visual phenotype or special growth requirements.

Donation Date: 2024-05-24

Donated by:  Cornell University Georg Jander

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Genus: Erysimum     Species: cheiranthoides

Seed type: Transgenic

Mutagen: CRISPR

Background: Elbtalaue

Segregation status: homozygous

Growth requirement: Stratification for 2 days at 4C recommended

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • 3bhsd-2


5beta-cardenolide synthesis reduced, accumulation of delta5-cardenolides


Younkin GC, Alani ML, Züst T, Jander G.; 2024-04-11; Four enzymes control natural variation in the steroid core of Erysimum cardenolides.; bioRxiv; PubMed ID: 38645095