NASC Stock Detail Page
NASC ID: N560780
Name: SALK_060780
ABRC stock number: SALK_060780
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_060780.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G03160.1=UTR3 and AT1G03170.1=P355 (at chr 1 pos 766900 (W/766824-766900) on the TAIR10). The sequences' 1-77 bps mapped on the genome. Pools P 35, R 32, C 31 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO392560. SALKseq_060780.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G27700.2=P355 and AT3G27710.1=P355 (at chr 3 pos 10262453 (C/10262453-10262518) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 35, R 32, C 31 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO395910. SALKseq_060780.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G11430.1 (at chr 3 pos 3595417 (W/3595356-3595417) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 35, R 32, C 31 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO399252. SALKseq_060780.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G12080.1 (at chr 3 pos 3850928 (C/3850928-3851005) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools: P 35, R 32, C 31 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO402594. SALKseq_060780.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G18340.1=P355 and AT5G18350.1 (at chr 5 pos 6073795 (W/6073766-6073795) on the TAIR10). The sequences' 1-30 bps mapped on the genome, 37-78 bps onto the T-DNA. Pools P 35, R 32, C 31 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO405922.
Donation Date: 2002-04-30
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_060780
Part of : SALK lines
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.