NASC

NASC ID: N575841

Name: SALK_075841

ABRC stock number: SALK_075841

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - FCAALL.166 R300-5'. At4g14385. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_075841.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G14385.1 (at chr 4 pos 8288732 (C/8288732-8288764) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 45-70 bps onto the T-DNA. Pools P 62, R 62, C 67 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400005.

SALKseq_075841.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G41380.1=UTR3 and AT2G41390.1=P355 (at chr 2 pos 17255973 (W/17255913-17255973) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 62, R 62, C 67 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389966.

SALKseq_075841.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G27360.1 (at chr 5 pos 9657661 (W/9657623-9657661) on the TAIR10). The sequences' 1-39 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 62, R 62, C 67 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403346.

SALKseq_075841.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G02240.1=UTR3 and AT3G02242.1 (at chr 3 pos 419897 (C/419897-419959) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 62, R 62, C 67 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393320.

SALKseq_075841.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G24080.2=P355 and AT3G24090.1 (at chr 3 pos 8697731 (W/8697691-8697731) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 62, R 62, C 67 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO396656.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_075841

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N654269 (SALK_075841C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G41380 AT2G41390 AT3G02240 AT3G02242 AT3G24080 AT3G24090 AT5G27360 At4g14385

EMBL accession:

BH852983

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.