NASC

NASC ID: N585428

Name: SALK_085428

ABRC stock number: SALK_085428

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At5g57330 MJB24.14 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_085428.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G68880.1=P355 and AT1G68890.1=P355 (at chr 1 pos 25896164 (C/25896164-25896217) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 77, R 74, C 77 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428169.

SALKseq_085428.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G28300.1 (at chr 1 pos 9897502 (C/9897502-9897557) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 77, R 74, C 77 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431484.

SALKseq_085428.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G57330.1=UTR3 and AT5G57340.1=P355 (at chr 5 pos 23222987 (C/23222987-23223050) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 77, R 74, C 77 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434791.

SALKseq_085428.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G64360.1 (at chr 5 pos 25737838 (W/25737796-25737838) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 77, R 74, C 77 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438095.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_085428

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_085428C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G28300 AT1G68880 AT1G68890 AT5G57340 AT5G64360 At5g57330

EMBL accession:

BZ594946

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.