NASC Stock Detail Page
NASC ID: N590923
Name: SALK_090923
ABRC stock number: SALK_090923
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_090923.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G59440.1=UTR3 and AT3G59450.1 (at chr 3 pos 21971458 (W/21971381-21971458) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 80, R 72, C 74 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425837. SALKseq_090923.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G33590.1 (at chr 2 pos 14225652 (W/14225575-14225652) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 80, R 72, C 74 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419204. SALKseq_090923.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G33600.1 (at chr 2 pos 14228616 (C/14228616-14228671) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 80, R 72, C 74 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422520. SALKseq_090923.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G26742.2=P355 and AT5G26740.2=P355 (at chr 5 pos 9289360 (C/9289360-9289416) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 80, R 72, C 74 N 72. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO429150.
Donation Date: 2002-01-09
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_090923
Part of : SALK lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.