NASC Stock Detail Page
NASC ID: N591045
Name: SALK_091045
ABRC stock number: SALK_091045
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. August 2002 TAIR gene annotation - T21L14.7 L300-5', At2g32990. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_091045.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G41810.1=P355 and AT2G41820.1=P355 (at chr 2 pos 17445187 (W/17445120-17445187) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 76, R 80, C 73 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432486. SALKseq_091045.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G23420.1=UTR3 and AT2G23430.1=UTR3 (at chr 2 pos 9976479 (C/9976479-9976540) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 76, R 80, C 73 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435793. SALKseq_091045.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G40130=P355 and AT5G40140.1 (at chr 5 pos 16057004 (C/16057004-16057083) on the TAIR10). The sequences' 1-80 bps mapped on the genome, 76-95 bps onto the T-DNA. Pools P 76, R 80, C 73 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO439097. SALKseq_091045.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G59510.1=UTR3 and AT5G59520.1=UTR3 (at chr 5 pos 23990882 (W/23990817-23990882) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 76, R 80, C 73 N 73. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419225.
Donation Date: 2002-01-09
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_091045
Part of : SALK lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.