NASC

NASC ID: N637031

Name: SALK_137031

ABRC stock number: SALK_137031

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At1g30420 T4K22.1 E xon T4K22.1 E xon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_137031.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G30420.1 (at chr 1 pos 10754923 (C/10754923-10754986) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 69, R 62, C 64 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO380810.

SALKseq_137031.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G18690.2=UTR3 and AT2G18700.1 (at chr 2 pos 8107064 (C/8107064-8107122) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 69, R 62, C 64 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO384152.

SALKseq_137031.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G18700.1 (at chr 2 pos 8111859 (W/8111782-8111859) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 69, R 62, C 64 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387502.

SALKseq_137031.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G05010.1 and AT4G05018.1 (at chr 4 pos 2568904 (W/2568854-2568904) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 69, R 62, C 64 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390843.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_137031

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664110 (SALK_137031C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G18690 AT2G18700 AT4G05010 AT4G05018 At1g30420

EMBL accession:

BZ385282

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.