NASC Stock Detail Page
NASC ID: N637079
Name: SALK_137079
ABRC stock number: SALK_137079
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.
Associated Polymorphisms:
SALKseq_137079.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G68450.1 (at chr 1 pos 25662003 (W/25661946-25662003) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 54-77 bps onto the T-DNA. Pools P 37, R 35, C 40 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO384154.
SALKseq_137079.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G27740.1=UTR3 and AT1G27750.1=P355 (at chr 1 pos 9656312 (W/9656254-9656312) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 37, R 35, C 40 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387504.
SALKseq_137079.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G34160.1=UTR3 and AT4G34170.1 (at chr 4 pos 16359644 (C/16359644-16359721) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 37, R 35, C 40 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390845.
SALKseq_137079.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G39050.1=UTR3 and AT5G39060.1 (at chr 5 pos 15636209 (W/15636148-15636209) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 37, R 35, C 40 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO394198.
SALKseq_137079.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G48510.1 and AT5G48515.1 (at chr 5 pos 19659496 (C/19659496-19659553) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 37, R 35, C 40 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397538.
Donation Date: 2003-03-11
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_137079
Part of : SALK lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.