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The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N66068

Name: mSPL13-2D

ABRC stock number: CS66068

Description: The SPL13 gene including the 1.3 kb upstream regulatory region was amplified from genomic DNA of Arabidopsis thaliana ecotype Col-0. Mutations at the miR156 target site were generated by site overlap extension PCR mutagenesis (Ho et al., 1989). PCR products were cloned into pCAMBIA1301 and Col-0 plants were transformed by floral dip method. Transgenic plants carry Hygromycin resistance.

Donation Date: 2010-08-18

Donated by:  Oregon State University Hiro Nonogaki

Donor Number: mSPL13-2D

Stock type: individual line

Material type: seed

Comment: Donors: Hiro Nonogaki. Parent Line: CS60000

Status Price (£)
Available £11.00

Germplasm Info

Seed type: Transgenic

Background: Col-0 (N60000)

Growth requirement: none

Phenotype

Slightly delayed leaf development.