NASC Stock Detail Page
NASC ID: N69081
Name: leb-1 GFP-h
ABRC stock number: CS69081
Description: leb-1 is a long ER body mutant isolated from EMS-mutagenized GFP-h plants. GFP-h is an Arabidopsis (ecotype Col) transformed with a pro35S:SP-GFP-HDEL gene encoding SP-GFP-HDEL, which is composed of the signal peptide of pumpkin (Cucurbita maxima) 2S albumin, and GFP followed by a 12 amino-acid sequence including an ER-retention signal, HDEL. The construct was inserted into the pBI121 binary vector. Homozygous for the mutation. Homozygous for the transgene.
Donation Date: 2015-12-15
| Donated by: | Kyoto University Ikuko Hara-Nishimura |
Donor Number: leb-1 GFP-h
Stock type: individual line
Material type: seed
Comment: Donors: Ikuko Hara-Nishimura. Clone: pro35S:SP-GFP-HDEL.
| Status | Price (£) |
|---|---|
| Currently unavailable | £11.00 |
Germplasm Info
Seed type: Mutant
Background: Col(Columbia)
Growth requirement: none
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
The amounts of the PYK10 protein were decreased in the leb-1 mutant. The leb-1 mutant has larger and fewer ER bodies.
References
Nagano, A.J. et al. 2009. Quantitative analysis of ER body morphology in an Arabidopsis mutant. Plant Cell Physiology 50(12):2015-22.PubMed ID: 19906837