NASC

NASC ID: N69206

Name: cvp1-3

ABRC stock number: CS69206

Description: Isolated from a population of EMS-mutagenized seedlings in a screen for vascular pattern mutants. Backcrossed 3 times to Col. Homozygous for the mutation. The cvp1-3 allele contains a missense mutation in the initiation codon, resulting in a null allele. Primer sequences: cvp1-3 (F14MT1, 5 -GGTCTTCCTCACTCTTAACG-3 ; F14MT2, 5 -GCACCGTCGAAACTGTTGTC-3 ).

Donation Date: 2016-05-13

Donated by:

Yale University Timothy Nelson Yale University Francine Carland

Donor Number: cvp1-3

Stock type: individual line

Material type: seed

Comment: Donor names: Francine M. Carland, Timothy Nelson. Associated polymorphisms: cvp1-3,

Status	Price (£)
Available	£11.00

Germplasm Info

Seed type: EMS mutant point mutation

Mutagen: ethylmethane sulfonate

Background: Col(Columbia)

Pedigree: Backcrossed 3 times to Col

Growth requirement: none

Phenotype

Aberant vascular patterning phenotype in the cotyledons, specifically a discontinuous venation pattern, reduction of vein loops, and thicker veins. Additional phenotypes include: Smaller, spoon-shaped cotyledons and retarded growth with a compact rosette composed of small vegetative leaves that fail to expand to wildtype size. Reduced plant height and apical dominance, delayed flowering time and senescence; serrated floral organs, shorter siliques and reduced fertility.

References

Carland, F.M. et al; 1999-11-11; Genetic regulation of vascular tissue patterning in Arabidopsis; Plant Cell; 11; 11; 2123-37; PubMed ID: 10559439

Carland, F.M. et al; 2002-09-01; The identification of CVP1 reveals a role for sterols in vascular patterning; The Plant Cell; 14; 9; 2045-58; PubMed ID: 12215504

Carland, F. et al; 2010-06-01; The sterol methyltransferases SMT1, SMT2, and SMT3 influence Arabidopsis development through nonbrassinosteroid products; Plant Physiology; 153; 2; 741-56; PubMed ID: 20421456