NASC Stock Detail Page
NASC ID: N69670
Name: pTIR1:tir1D170E/M473L-Myc #10-10 AXR3NT-GUS
ABRC stock number: CS69670
Description: The pTIR1:tir1-Myc construct was made by introducing a 2-kb 5 upstream region of the TIR1 gene adjacent to the tir1 cDNA harboring the D170E and M473L mutations into pGWB16 vector and transformed into the AXR3NT-GUS (Col-0) background (CS69686). Line 6-10 (CS69669) is slightly stronger than line 10-10. Hygromycin resistant. Homozygous for the transgene.
Donation Date: 2016-11-09
| Donated by: | University of California San Diego Mark Estelle University of California San Diego Mike Prigge |
Donor Number: pTIR1:tir1D170E/M473L-Myc #10-10 AXR3NT-GUS
Stock type: individual line
Material type: seed
Comment: Donor names: Mike Prigge, Mark A. Estelle. Clone name: pTIR1:tir1D170E/M473L-Myc, HS::AXR3NT-GUS
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Protein fusion
Background: Col (N69686)
Growth requirement: none
Phenotype
More lateral roots and smaller rosette leaves.
References
Yu, H. et al; 2013-05-01; Mutations in the TIR1 auxin receptor that increase affinity for auxin/indole-3-acetic acid proteins result in auxin hypersensitivity; Plant Physiology; 162; 1; 295-303; PubMed ID: 23539280