NASC

NASC ID: N73560

Name: pLH13 in as2-7D

ABRC stock number: CS73560

Description: Transgenic line carrying fluorescent membrane marker pLH13 (p35S::mCitrine-RCI2A) in an as2-7D mutant background. The as2-7D allele has a G to A mutation at base 1,484 upstream of the ATG translational start site of AT1G65620. The transgene pLH13 consists of mCitrine tagged to RARE-COLD-INDUCIBLE 2A (RCI2A) protein driven by the CaMV 35S promoter. The membrane marker silences frequently, and hence requires screening in each generation.

Donation Date: 2024-05-24

Donated by:

Cornell University Adrienne Roeder Zhejiang University Lilan Hong

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Genus: Arabidopsis     Species: thaliana

Seed type: Transgenic

Mutagen: ethylmethane sulfonate

Segregation status: homozygous

Growth requirement: none

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
as2-7D			AT1G65620

Phenotype

The sepals exhibit folds and outgrowths on the outer epidermis, while the inner epidermis is smooth. The cotyledons exhibit upward leaf curving. The older vegetative leaves show some degree of upward curving as well. The mutant also exhibits late flowering.

References

Yadav AS, Hong L, Klees PM, Kiss A, Petit M, He X, Barrios IM, Heeney M, Galang AMD, Smith RS, Boudaoud A, Roeder AHK.; 2024-04-08; Growth directions and stiffness across cell layers determine whether tissues stay smooth or buckle.; bioRxiv; PubMed ID: 37546730