NASC Stock Detail Page
NASC ID: N76502
Name: Alonso Crosby and Ecker
ABRC stock number: CS76502
Description: First set of 10,000 T-DNA lines produced by J. Alonso, W. Crosby and J. Ecker; packaged as 100 pools of 100 lines. These insertion lines were generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens. The vector employed was pROK2 (Baulcombe et al., 1986, Nature 321:446). Each T1 transformant was grown individually, and equal quantities of T2 seeds combined to create pools of 10 lines. These pools of seeds were grown at ABRC to generate the T3 seed pools which are the distributed stocks. The parental line (N60000) is included with this stock.
Donation Date: 1999-12-07
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker University of Windsor William Crosby North Carolina State University Jose M. Alonso |
Part of : Alonso Crosby and Ecker
Stock type: set (View set contents)
Material type: seed
| Status | Price (£) |
|---|---|
| Currently unavailable | £107.00 |
Germplasm Info
Genus: Arabidopsis Species: thaliana
Mutagen: ema
Background: Col-0(N60000)
Transformation method: These insertion lines were generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens. The vector employed was pROK2 (Baulcombe et al., 1986, Nature 321:446). Each T1 transformant was grown individually, and equal quantities of T2 seeds combined to create pools of 10 lines.
Ecotype data
Location: Columbia
Country: USA
Altitude Min: 1
Altitude Max: 100
Latitude Min: 38.3
Latitude Max: 38.3
Longitude Min: -92.3
Longitude Max: -92.3
Monthly Preciptation Spring Min: 60
Monthly Preciptation Spring Max: 70
Monthly Preciptation Autumn Min: 30
Monthly Preciptation Autumn Max: 40