NASC

NASC ID: N851071

Name: WiscDsLox319C09

ABRC stock number: CS851071

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox319C09 .

Wiscseq_DsLox319C09.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G60210.1 (chr 5 pos 24245986 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG798615.

Wiscseq_DsLox319C09.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G32590.1 (at chr 2 pos 13830029 (C/13830029-13830113) on the TAIR10). The sequences' 1-85 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 1, R 9, C 7 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452113.

Wiscseq_DsLox319C09.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G33070.1-P355 and AT4G33080.1-P355 (chr 4 pos 15959500 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG798614.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox319C09

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox319C09	WiscDsLox319C09	AT2G32590 AT4G33070 AT4G33080 At5g60210