NASC Stock Detail Page
NASC ID: N16562
Name: Education kit -Gibberellic Acid Physiology and Plant Transformation
ABRC stock number: CS16562
Description: This set of three seed stocks and two DNA stocks can be used with the "Gibberellic Acid Physiology and Plant Transformation" module to introduce GA (gibberellic acid) hormone physiology and plant transformation in an undergraduate laboratory module. In this module, Arabidopsis ga5 semi-dwarf mutant plants with reduced activity of GA 20-oxidase are transformed with a construct containing the GA5 gene. The dwarf mutant plants transformed with a wild-type version of the gene show normal size, demonstrating successful complementation of the mutant phenotype. Besides visual observation of the phenotype, students learn additional techniques, including PCR and agarose gel electrophoresis. This experiment is cost effective and can be completed within a 4-month term.
Donation Date: 2011-02-08
| Donated by: | Arabidopsis Biological Resource Center |
Part of : Teaching kits for Arabidopsis
Stock type: set (View set contents)
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £0.00 |
Germplasm Info
References
Molina, I. et al; 2009-05-01; Transformation of a dwarf Arabidopsis mutant illustrates gibberellin hormone physiology and the function of a Green Revolution gene; Biochemistry and molecular biology education; 37; 3; 107-7; PubMed ID: 21567727