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Genetics | Seed irradiation and sector analysis | Verification of the CAUT lines

Background and theory.

The ch-42 allele on chromosome 4 (red) is inactive but the wild-type CH-42 function is provided by the T-DNA insert (yellow) on another chromosome (blue). The plant is initially heterozygous for the recessive gene b with the wild-type copy (B) linked in cis to the T-DNA. After irradiation (lower panel) phenotypically yellow sectors are selected and these have lost both the CH-42 copy on the T-DNA and the adjacent B gene. The recessive b mutant is the only copy and the tissue is both yellow and genotypically mutant. The analysis of phenotype of the sector and adjacent wild-type tissue can be used to look for autonomous and non-autonomous interactions.